Confocal microscopy


Confocal microscopy is one of the methods of optical microscopy that has significant contrast compared to classical microscopes due to the use of a diaphragm that cuts off the flow of background scattered light. In a confocal microscope, an image of one point of an object is recorded at each moment of time, and a full image is constructed by scanning (moving the sample or rearranging the optical system). In order to register light from only one point, a small diaphragm is located after the objective lens in such a way that the light emitted by the analyzed point passes through the diaphragm and will be registered, and the light from the remaining points is mainly delayed by the diaphragm.